Extracellular matrix (ECM) components are critical for all aspects of cell proliferation, adhesion,\nand morphological alteration. Recent progress has yielded multiple molecular drugs that specifically\ntarget gene products which are expressed at high levels in tumor cells. We investigated\nwhether the sensitivity of tumor cells to molecular target drugs could be altered when cells were\ncultured on surfaces with various coating conditions such as lysine, laminin, Matrigel, collagen\ntype I, and human fibronectin (HFN). This study evaluates the IC50 values of imatinib in oral\nsquamous cell carcinoma (OSCC) cell lines when cells are cultured on plates coated with ECM\ncomponents such as collagen type I and HFN. Four OSCC cell linesââ?¬â?SQUU-A, SQUU-B, SAS, and NAââ?¬â?\nare used. Cell proliferation was assessed using WST-8 reagent. Collagen type I and HFN significantly\nenhanced OSCC cell proliferation compared with control. Imatinib cytotoxicity was demonstrated\nfollowing culture of OSCCs in culture plates coated with collagen type I or HFN. However,\nthere were no significant changes in imatinib IC50 values between collagen type I and HFN. These\nresults indicate that some molecular target drugs exhibit cancer cell cytotoxicity without being influenced\nby cell environment factors such as the ECM. These results may aid in the search for molecular\ntarget drugs to apply in the clinical chemotherapy of OSCC.
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